CYP450 Protein Assay - Human Induction Kit

The CYP450 Protein Assay – Human Induction Kit, uses an LC/mass spectrometry workflow to enable direct measurement of protein expression changes of individual CYP450 isoforms with high specificity, sensitivity, and accuracy. The kit has been developed to employ MRM analysis to quantify the protein levels of four key CYP450 protein isoforms for induction studies: 1A2, 2B6, 3A4, and 3A5.

Product Name Part Number Quantity/
Package
p450 Human Induction Starter Kit
The AB SCIEX P450 Human Induction Starter Kit provides a complete package for the analysis of four human CYP450 isoforms (1A2, 2B6, 3A4, and 3A5) using MRM analysis on an AB SCIEX QTRAP® or Triple Quad™ system. The kit contains a detailed protocol, instrument data acquisition methods, processing methods, LC column, stable isotope labeled synthetic peptide standards, trypsin, and all buffers and reagents for 100 assays.		 4445494 1 kit
p450 Human Induction 100 Assay Kit
The AB SCIEX P450 Human Induction 100 Assay Kit provides a complete protocol, instrument data acquisition methods, processing methods and stable isotope labeled synthetic peptide standards for the analysis of four human CYP450 isoforms (1A2, 2B6, 3A4, and 3A5) for induction studies using LC/MS/MS on an AB SCIEX QTRAP® or Triple Quad™ system. Kit contains enough reagents for 100 Assays. Must order Trypsin TPCK (500 ug) and Peptide C18 Column separately.		 4445252 1 kit
Trypsin TPCK (500 µg)
A modified (TPCK treated) serine protease that specifically hydrolyzes peptide bonds at the carboxyl side of lysine and arginine residues. Modified trypsin is treated with N-tosyl-L-phenylalanine chloromethyl ketone (TPCK) to inactivate extraneous chymotryptic activity. For use with the P450 Human Induction 100 Assay Kit. Ten tubes are included in each package and each tube will be sufficient for at least 10 samples (1 mg of protein digested with a 1:20 ratio = 50 μg). 		4445250 10 tubes
Peptide C18 Column
A reversed phase C18 column specifically designed for the analysis of CYP450 isoforms by LC/MS/MS. Good for at least 200 analyses.		 4445251 1 column

To order, contact your local Sales Representative or Sales Office.

Product Description

  • Directly measure CYP450 induction at the protein level without expensive antibodies
  • Measure individual CYP450 isoforms with high specificity, sensitivity, and accuracy
  • Easy-to-use kits and method employ a common MRM workflow
  • Results are equivalent to traditional mRNA or activity assays
  • Everything You Need to Perform Protein QuantitationThe Starter kit contains peptide standards, sample preparation reagents and buffers, trypsin, a column, data acquisition methods, and processing methods and templates for performing protein quantitation using MRM analysis on an AB SCIEX Triple Quad™ or QTRAP® system. The peptide standards for each protein are synthesized using isotopically enriched amino acids (lysine and arginine) to provide the highest quantitative robustness. The LC/MS/MS method allows for a direct measurement of CYP protein levels without the use of antibodies, providing savings in both time and expense (Figure 1).
    Figure 1:Example MRM Datacms_075272
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  • Advanced Data Analysis SoftwareData is processed with MultiQuant™ Software. The software automatically integrates the MRM data and computes area ratios to the added peptide internal standards for all samples. Processed area ratio results are imported into a Microsoft Excel template for computation of protein level data. Results are plotted in a simple bar chart to compare the increase in expression of each CYP450 isoform in each sample as compared to the negative controls.
  • Correlation between enzymatic activity, mRNA expression, and protein expression (as measured by LC-MS/MS) is excellent (Figure 2). Both baseline and inducible levels of the CYP isoforms are easily detected by the LC-MRM protein quantitation method.
    Figure 2:Assay Type Comparisoncms_075274
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For Research Use Only. Not for use in diagnostic procedures.