aTRAQ™ Kits for Amino Acid Analysis of Hydrolysates
The aTRAQ™ Kits for Amino Acid Analysis of Hydrolysates enables you to quantitate 20 amino acids from protein hydrolysate samples more than 3 times faster than conventional amino acid analyzers. The kits are designed for use with LC/MS/MS
- Analysis is unaffected by chromatographic shifts due to column aging
- Stable reagents and derivatized samples
- Internal standards for every amino acid provide confident identification and superior accuracy and precision
- High throughput with an analysis time of 18 minute per sample
- Small sample requirement, 1 µg
- Wide linear dynamic range of greater than four orders of magnitude with an LLOQ and ULOQ of <100 fmole to >1 nmole, respectively on a 3200 QTRAP® system
- Complete kit that includes the required reagents, buffers, standards, controls, and column
- Ability to add custom amines, amino acids and small peptides and create corresponding internal standards
- Ability to automate pipetting steps (optional)
Status: Available 
Faster Performance. Better Accuracy. The aTRAQ™ Reagents are used to provide a consistent mass tag to aid in the MS/MS fragmentation of the sample and internal standard in a molecular weight region with reduced interference. This improves the precision and accuracy of quantitation by providing a mechanism to use an internal standard for each amino acid analyzed and provide an extra degree of confirmation by using the retention time and the molecular weight of the standard and sample for confirmation of each amino acid. Figure 1: General Labeling Reaction
Buffer is added to the dried hydrolysate to maintain a basic pH for the labeling reaction. The samples are labeled with aTRAQ™ reagent Δ8, allowed to react for 30 min, dried, and mixed with the internal standards pre-labeled with aTRAQ™ reagent Δ0. The two aTRAQ reagents are identical except for the number of isotopes they contain. This mixture is then injected for LC/MS/MS analysis using either a triple quadrupole or QTRAP® system operating in MRM mode. HPLC separation is performed using a C18 column heated to 50 °C. The gradient, wash, and equilibration take a total of 18 min (see Figure 2). The use of the Scheduled MRM™ Algorithm maximizes dwell time while monitoring of large numbers of MRM transitions, resulting in optimum data quality and reproducibility. Figure 2: Representative Chromatogram
Using this method, the same amino acid labeled with the different tags cannot be distinguished by HPLC since they have identical retention times. In the MS/MS mode the tags fragment to yield reporter ions that have been encoded with isotopes to yield different masses. MRM transitions made up of the labeled amino acid masses and the reporter ion masses are used to differentiate the same amino acid from the sample and the internal standard and the ratio of these peak areas is used to calculate the concentration of the amino acid in the sample (see Figure 3). Figure 3: General Scheme
What's Included in The System For more information on this system and its components, please contact your local sales representative.
For Research Use Only. Not for use in diagnostic procedures. Warranty: Standard parts and labor warranty for one year starting from the completion of instrument commissioning.
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