CYP450 Protein Assay - Human Induction Kit

Mass Spec Reagents-CYP450 Protein Assay - Human Induction Kit
Mass Spec Reagents-CYP450 Protein Assay - Human Induction Kit
Mass Spec Reagents-CYP450 Protein Assay - Human Induction Kit
Mass Spec Reagents-CYP450 Protein Assay - Human Induction Kit
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The CYP450 Protein Assay - Human Induction Kits, use an LC/mass spectrometry workflow to enable direct measurement of protein expression changes of individual CYP450 isoforms with high specificity, sensitivity, and accuracy. These kist have been developed to employ MRM analysis to quantify the protein levels of  seven key CYP450 protein isoforms for induction studies: 1A2, 2B6, 2C9, 2C19, 2E1, 3A4 and 3A5.  Both a four isoform (1A2, 2B6, 3A4 and 3A5) and a seven isoform ( 1A2, 2B6, 2C9, 2C19, 2E1, 3A4 and 3A5) kit are available.

  • Directly measure CYP450 induction at the protein level without expensive antibodies
  • Measure individual CYP450 isoforms with high specificity, sensitivity, and accuracy
  • Easy-to-use kits and method employ a common MRM workflow
  • Results are equivalent to traditional mRNA or activity assays

 

 

 

Status: Available Item status information

 

Everything You Need to Perform Protein Quantitation The Starter kit contains peptide standards, sample preparation reagents and buffers, trypsin, a column, data acquisition methods, and processing methods and templates for performing protein quantitation using MRM analysis on an AB SCIEX Triple QuadTM or QTRAP® system. The peptide standards for each protein are synthesized using isotopically enriched amino acids (lysine and arginine) to provide the highest quantitative robustness. The LC/MS/MS method allows for a direct measurement of CYP protein levels without the use of antibodies, providing savings in both time and expense (Figure 1).

Figure 1: Example MRM Data

Example MRM Data-figure1

 

 

 

 

 

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Advanced Data Analysis Software Data is processed with MultiQuantTM Software. The software automatically integrates the MRM data and computes area ratios to the added peptide internal standards for all samples. Processed area ratio results are imported into a Microsoft Excel template for computation of protein level data. Results are plotted in a simple bar chart to compare the increase in expression of each CYP450 isoform in each sample as compared to the negative controls.
Correlation between enzymatic activity, mRNA expression, and protein expression (as measured by LC-MS/MS) is excellent (Figure 2). Both baseline and inducible levels of the CYP isoforms are easily detected by the LC-MRM protein quantitation method.

 

Figure 2: Assay Type Comparison

Assay Type Comparison-figure1

 

 

 

 

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For Research Use Only. Not for use in diagnostic procedures.