CYP450 Protein Assay - Human Induction Kit

Mass Spec Reagents-CYP450 Protein Assay - Human Induction Kit
Mass Spec Reagents-CYP450 Protein Assay - Human Induction Kit
Mass Spec Reagents-CYP450 Protein Assay - Human Induction Kit
Mass Spec Reagents-CYP450 Protein Assay - Human Induction Kit
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Protein expression changes of individual cytochrome P450 (CYP450) isoforms can be directly measured using the CYP450 Protein Assay – Human Induction Kits and an LC/mass spectrometry workflow for high specificity, sensitivity, and accuracy. These kits have been developed to employ multiple reaction monitoring (MRM) analysis to quantify seven CYP450 isoforms that are key proteins for induction studies: 1A2, 2B6, 2C9, 2C19, 2E1, 3A4 and 3A5.  Kits with varying numbers of isoforms are available: 1) the four-isoform kit (1A2, 2B6, 3A4 and 3A5), and 2) the seven-isoform kit (1A2, 2B6, 2C9, 2C19, 2E1, 3A4 and 3A5).

  • Directly measure CYP450 induction at the protein level without expensive antibodies
  • Measure individual CYP450 isoforms with high specificity, sensitivity, and accuracy
  • Easy-to-use kits and methodology employ a common MRM workflow
  • Results are equivalent to traditional mRNA or activity assays

 

 

 

Status: Available Item status information

     

    Everything You Need to Perform Protein Quantitation The Starter kit contains peptide standards, sample preparation reagents and buffers, trypsin, a column, data acquisition methods, and processing methods and templates for performing protein quantitation using MRM analysis on an AB SCIEX Triple Quad™ or QTRAP® system. The peptide standards for each protein are synthesized using isotopically enriched amino acids (lysine and arginine) to provide the highest quantitative robustness. The LC/MS/MS method allows for a direct measurement of CYP protein levels without the use of antibodies, providing savings in both time and expense (Figure 1).

    Figure 1: Example MRM Data

    Example MRM Data-figure1

     

     

     

     

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    Advanced Data Analysis Software Data is processed with MultiQuant™ Software. The software automatically integrates the MRM data and computes area ratios to the added peptide internal standards for all samples. Processed area ratio results are imported into a Microsoft Excel template for computation of protein level data. Results are plotted in a simple bar chart to compare the increase in expression of each CYP450 isoform in each sample as compared to the negative controls.
    Correlation between enzymatic activity, mRNA expression, and protein expression (as measured by LC-MS/MS) is excellent (Figure 2). Both baseline and inducible levels of the CYP isoforms are easily detected by the LC-MRM protein quantitation method.

     

    Figure 2: Assay Type Comparison

    Assay Type Comparison-figure1

     

     

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    For Research Use Only. Not for use in diagnostic procedures.