mTRAQ® Reagents

Mass Spec Reagent-mTRAQ® Reagents
Mass Spec Reagent-mTRAQ® Reagents
Mass Spec Reagent-mTRAQ® Reagents
Mass Spec Reagent-mTRAQ® Reagents
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The mTRAQ® Reagents are amine-specific, stable isotope labeled reagents, available in duplex or triplex format, designed for high confidence protein and peptide biomarker verification using MRM analysis on QTRAP® systems and the Triple Quadrupole Mass Spectrometers.

The use of the triplex chemistry will increase sample throughput by 100%.

  • Duplex or triplex mass difference tags (Non-Isobaric Amine Tags)
  • Established chemistry with > 95% labeling efficiency
  • High selectivity MRM based relative quantitation of proteins/peptides/PTMs
  • Supports Global Internal Standard workflow for robust reproducible quantitative MRM assays in biomarker verification
  • Compatible with fractionation
  • Easy transition from iTRAQ® reagent based discovery to verification.
  • Significantly more economical compared to stable isotope labeled synthetic peptide approach for biomarker verification when larger numbers of peptides need to be monitored
  • Supported by complete suite of software for biomarker verification, including ProteinPilotTM Software, MRMPilotTM Software, and MultiQuantTM Software

Status: Available Item status information

 

Innovative Peptide Tagging Chemistry The mTRAQ® Reagents are non - isobaric peptide labeling reagents that enable you to label all primary amines, regardless of peptide class. The reagents are an extension of the iTRAQ® Reagent technology, in that they are chemically identical (Fig 1). The peptides labeled with the different mTRAQ® Reagents have identical retention time and ionization characteristics but different masses (0, 4 or 8 amu mass difference triplex labeled Lys peptides and 0, 8, or 16 for Arg peptides). The MRM quantitation is performed using the non-isobaric sequence specific ions instead of reporter ions for higher specificity in complex mixtures.

Figure 1: Chemical Structure of mTRAQ® Reagents

Cliquid Drug Screen and Quant Homepage-figure1

 

 

 

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Figure 2: Global Internal Standard Workflow

Global Internal Standard Workflow-figure2

 

 

 

 

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High Confidence Biomarker Verification using Robust Methodology Obtain robust performance with a Global Internal Standard (GIS) methodology. The GIS is prepared by labeling pooled samples with mTRAQ® Reagents Δ8. This GIS is then spiked into individual samples labeled with either mTRAQ® Reagents Δ0 or mTRAQ® Reagents Δ4 before MRM analysis (Fig 2). This offers significant benefits such as:

  • Enables normalization and comparison across
    • Many patient samples
    • Entire duration of the project
    • Multiple instruments / multiple laboratories
  • Corrects for experimental inconsistencies
    • Injection inconsistencies
    • Changes in MS performance
    • Changes in LC performance
  • Most economical approach
    • Enables quantitation of large number of proteins and peptides by providing a more cost effective way to create internal standards
    • mTRAQ® reagents - triplex will double sample throughput

Complete Solution for Targeted Quantitative Proteomics One key advantage of being able to analyze hundreds of compounds a week is that you can quickly build a substantial database of optimization conditions. Each time you find an optimized set of conditions for a given compound, DiscoveryQuant software stores it in a networked database, which you can then use in assays at any time, in any location, with other mass spectrometers in other labs- a significant advantage for pharmaceutical companies in the age of globally connected data.

For Research Use Only. Not for use in diagnostic procedures.