mTRAQ® Reagents

Mass Spec Reagent-mTRAQ® Reagents
Mass Spec Reagent-mTRAQ® Reagents
Mass Spec Reagent-mTRAQ® Reagents
Mass Spec Reagent-mTRAQ® Reagents
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The mTRAQ® Reagents are amine-specific, stable-isotope-labeled reagents, available in duplex or triplex format, designed for high-confidence protein and peptide biomarker verification using multiple reaction monitoring (MRM) analysis on QTRAP® Systems and the Triple Quad™ LC/MS/MS Systems.

The use of this triplex chemistry will increase sample throughput by 100%.

  • Duplex or triplex mass-difference tags (non-isobaric amine tags) available
  • Established chemistry with >95% labeling efficiency
  • High-selectivity, MRM-based, relative quantitation of proteins/peptides/PTMs
  • Global Internal Standard (GIS) workflow is supported for robust, reproducible, quantitative MRM assays in biomarker verification
  • Fractionation-compatible reagent
  • Easy transition from protein discovery based on labeling with iTRAQ® Reagents to verification
  • Significantly more economical approach compared to use of stable-isotope-labeled synthetic peptides for biomarker verification when moitoring larger numbers of peptides
  • Biomarker verification supported by a complete suite of software, including ProteinPilot™, MRMPilot™, and MultiQuant™ Software

Status: Available Item status information

 

Innovative Peptide Tagging Chemistry The mTRAQ® Reagents are non - isobaric peptide labeling reagents that enable you to label all primary amines, regardless of peptide class. The reagents are an extension of the iTRAQ® Reagent technology, in that they are chemically identical (Fig 1). The peptides labeled with the different mTRAQ® Reagents have identical retention time and ionization characteristics but different masses (0, 4 or 8 amu mass difference triplex labeled Lys peptides and 0, 8, or 16 for Arg peptides). The MRM quantitation is performed using the non-isobaric sequence specific ions instead of reporter ions for higher specificity in complex mixtures.

Figure 1: Chemical Structure of mTRAQ® Reagents

Cliquid Drug Screen and Quant Homepage-figure1

 

 

 

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Figure 2: Global Internal Standard Workflow

Global Internal Standard Workflow-figure2

 

 

 

 

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High Confidence Biomarker Verification using Robust Methodology Obtain robust performance with a Global Internal Standard (GIS) methodology. The GIS is prepared by labeling pooled samples with mTRAQ® Reagents Δ8. This GIS is then spiked into individual samples labeled with either mTRAQ® Reagents Δ0 or mTRAQ® Reagents Δ4 before MRM analysis (Fig 2). This offers significant benefits such as:

    • Enables normalization and comparison across
      • Many patient samples
      • Entire duration of the project
      • Multiple instruments / multiple laboratories
    • Corrects for experimental inconsistencies
      • Injection inconsistencies
      • Changes in MS performance
      • Changes in LC performance
    • Most economical approach
      • Enables quantitation of large number of proteins and peptides by providing a more cost effective way to create internal standards
      • mTRAQ® reagents - triplex will double sample throughput

    Complete Solution for Targeted Quantitative Proteomics One key advantage of being able to analyze hundreds of compounds a week is that you can quickly build a substantial database of optimization conditions. Each time you find an optimized set of conditions for a given compound, DiscoveryQuant software stores it in a networked database, which you can then use in assays at any time, in any location, with other mass spectrometers in other labs- a significant advantage for pharmaceutical companies in the age of globally connected data.

    For Research Use Only. Not for use in diagnostic procedures.