Biologic Peptide Mapping Analysis

Fast Biologic Peptide Mapping Analysis for Complete Coverage

Whether you are involved in biopharma discovery, early development or late development, you need fast and dependable peptide mapping analysis techniques that you can count on.

In early development candidate selection, determining the appropriate critical quality attributes (CQA) is paramount and depends on extremely complex data processing and analysis. In late development, throughput is your top priority so you can process and analyze high sample volumes, quickly. Although the requirements of peptide mapping analyses change during the development pipeline, the need to store, access and share large data sets is consistent throughout.

With SCIEX mass spec solutions, you can now benefit from 100% sequence coverage in a single injection and see low level post-translational modifications – faster than ever before.

Solving the Challenges

SCIEX combines cutting edge mass spectrometry technology into an integrated solution designed specifically to address the challenges of peptide mapping at all stages of the therapeutic development pipeline.

Comprehensive Sequence Analysis with CESI-MS
Experience the orthogonal separation technique of CESI-MS, providing 100% sequence coverage with a single injection, from a single protease digestion.

Peptide Analysis Becomes Routine with the X500B QTOF
Simplify and accelerate peptide mapping workflows with the powerful and intuitive X500B QTOF system that eliminates complex method development by using a generic data-independent SWATH® Acquisition method for the analysis of all detectable peptides in a sample.

Answers at the Speed of Light
Eliminate time consuming manual data analysis, interpretation and reporting with revolutionary BioPharmaView™ Software. Process large batches, annotate the peptide sequence and report detailed quantitation information – it’s automated and accurate.

Peptides Have Nowhere to Hide – See More, Faster

CESI-MS has exceptional separation efficiency for peptides (especially the very short or very long, or highly charged peptides) and glycopeptide mapping of mAbs. It can differentiate between Asp and IsoAsp deamidation and other PTMs, with the exceptional ability to provide 100% coverage from a single enzyme digestion in a single analysis.

Peptide Mapping Becomes Routine, Transform Your Workflows

The powerful X500B QTOF System combined with the intuitive SCIEX OS point-and-click icon based interface has revolutionized routine peptide analysis. Dramatically reducing the complexity of setting up LC-MS methods and batches, the system makes standardized peptide mapping analyses accessible to scientists of any skill level, without the involvement of mass spec experts. The use of SWATH Acquisition, an unbiased data-independent acquisition strategy, requires no up-front method development but can provide comprehensive sample coverage for all detectable peptides in a single injection.

Unbeatable Data Processing for Extremely Fast Data to Answers

Now you can get faster answers to your peptide mapping questions with the powerful data processing capabilities of BioPharmaView Software. Combined with the accurate mass data from the X500B QTOF or TripleTOF® systems, it is a rapid and intuitive package for analyzing your peptide mapping data.

Webinar - Routinely Identify Low-Level Attributes- Fast and Accurately

Solutions and Offerings

Simple and Streamlined LC-MS for Biologic Peptide Mapping

For biopharma development labs that need to do standardized peptide mapping analyses, the X500B QTOF System offers an easy-to-use platform that is accessible to all user levels.

ExionLC™ AD

Delivers high accuracy, reliability and repeatability across thousands of injections, with maximum uptime

X500B QTOF System

High speed accurate mass analysis with the compact and streamlined QTOF System and the renowned Turbo V™ ionization source

SCIEX OS

Intuitive point-and-click acquisition software makes it easier than ever to set-up and run your methods

BioPharmaView™ Software

Automated biotherapeutic data processing for accurate peptide identification, including post-translation modifications

Biologics LC-MS Training

Get up and running quickly with access to our online training programs specifically for biotherapeutic characterization

Technical Note

Comparative Peptide Mapping of trastuzumab

Technical Note

Automating Quantitation of Oxidation Sites in mAb Characterization

Solutions Guide

Peptide Mapping Analysis

Case Study

Peptide Mapping Case Study

Method Brief

Non-reduced Biotherapeutic Peptide Mapping

Method Brief

Biotherapeutic Peptide Mapping: IDA Method

Method Brief

Biotherapeutic Peptide Mapping: SWATH Acquisition Method

In-depth Characterization by CESI-MS

For labs that need to perform in-depth biotherapeutic characterization, the increased sensitivity provided by CESI high resolution CE separation and enhanced dynamic range of the TripleTOF 6600 system can help you get the advanced answers you need.

CESI 8000 Plus

In-depth characterization of digested biologic product—especially short peptides, long hydrophobic peptides, drug-linked peptides, glycopeptides and deamidated peptides.

TripleTOF 6600

Maximal sensitivity and broad linear quantitation range for large molecules, ideal for in-depth characterization and detection of low-level peptide species.

BioPharmaView Software

Automated biotherapeutic data processing for accurate peptide identification, including post-translation modifications

Technical Note

Rapid Characterization of Biologics using CESI and TripleTOF systems

Webinar

Multilevel Characterization of Biotherapeutics using CESI-MS: from intact to peptide mapping

Webinar

Intact Proteoform Characterization using CESI-MS

Webinar

CESI-MS for Biotherapeutic Characterization

Technical Note

Fast, Efficient Disulfide Bond Mapping Using BioPharmaView Software

Technical Note

Automated High-throughput Trypsin Digests for Peptide Mapping Analysis

Software Bulletin

BioPharmaView™ Software Bulletin

 

Learning Center    
Technical Note: Comparative Peptide Mapping of trastuzumab Download  
Technical Note: Automating Quantitation of Oxidation Sites in mAb Characterization Download  
Solutions Guide: Peptide Mapping Analysis Download  
Case Study: Peptide Mapping Case Study Download  
Method Brief: Non-reduced Biotherapeutic Peptide Mapping Download  
Method Brief: Biotherapeutic Peptide Mapping: IDA Method Download  
Method Brief: Biotherapeutic Peptide Mapping: SWATH Acquisition Method Download  
Technical Note: Rapid Characterization of Biologics using CESI and TripleTOF systems Download  
Webinar: Multilevel Characterization of Biotherapeutics using CESI-MS: from intact to peptide mapping Watch  
Webinar: Intact Proteoform Characterization using CESI-MS Watch  
Webinar: CESI-MS for Biotherapeutic Characterization Watch  
Technical Note: Fast, Efficient Disulfide Bond Mapping Using BioPharmaView Software Download  
Technical Note: Automated High-throughput Trypsin Digests for Peptide Mapping Analysis Download  
Software Bulletin: BioPharmaView™ Software Bulletin Download