Recommended N-Glycan MALDI Sample Preparation Matrices for TOF/TOF Instruments


Date: 10/18/2018
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Depending on what type of N-glycan moiety that is being analyzed on a TOF/TOF system, it is recommended that certain MALDI sample preparation techniques, matrices and acquisition methods be used (see table):

Note: Matrix still works the best if it is freshly recrystallized, even if this type of sample prep is a bit more time-consuming.

Carbohydrate type

TOF/TOF system acquisition recoommendations

Neutral glycans or reductive aminated glycans

Positive reflector mode and positive ion MS/MS; DHB recrystallized with MeOH or EtOH as a matrix

 

Acidic glycans (e.g., phosphorylated/sulfated/sialylated to a certain extent), 2-AA-labelled glycans

 

Negative reflector mode for acidic glycan; Sialic acid containing glycans as a matrix in positive linear ion mode due to the high fragmentation in reflector mode 

 

2-AB labelled glycans and permethylated glycans

 

Positive or negative reflector mode and positive ion MS/MS; DHB recrystallized with MeOH or EtOH or THAP as matrices, perhaps HCCA for large OS

Positive or negative reflector or linear mode and positive ion MS/MS; DHB with ACN/water as a matrix

O-glycans or glycosaminoglycans

Positive reflector mode and positive ion MS/MS; DHB recrystallized with MeOH or EtOH as a matrix

 

Glycopeptides

 

Positive reflector mode and positive ion MS/MS; HCCA and CAN as matrices