Amplifex Diene Reagent

Benefit from an increased shelf life with the new solid formulation

For research use only. Not for use in diagnostic procedures.

Signal enhancement maximized through intelligent chemistry

Amplifex Diene reagent is designed specifically to enhance signal strength up to 1000-fold (depending on the analyte) and can be used to specifically derivatize any molecule with a cis-diene system, such as vitamin D3, vitamin D2 and their analogs.

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With the Amplifex Diene reagent, you can drastically reduce sample size and achieve ultra-low LLOD and LLOQ levels that were difficult, if not impossible, to achieve by mass spectrometry even on the most-sensitive platforms.

  • Intelligent design boosts ionization efficiency and improves fragmentation
  • Substantial reduction in sample volume and analysis time
  • Reactions are simple (one-step derivatization) and fast (30 minutes)
  • Generic derivatization technique can be used with any molecule that has a cis-diene system, such as a broader range of vitamin D3 and vitamin D2 analogs that are natural or synthetic
  • Now provided as a solid reagent for an increase of over 30% in shelf life compared to the previous liquid formulation

Amplifex Diene reagent is a simple, quick chemistry that improves ionization and enhances sensitivity for any cis-diene system, enabling workflows that push the limits of sample volume.

Signal enhancement for molecules with cis-diene functionality


1,25-dihydroxy vitamin D3 derivatized withAmplifex Diene reagent compared to underivatized provides a ~165-fold signal enhancement.

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Signal enhancement for other molecules with cis-diene functionality that have been derivatized with Amplifex Diene reagent.

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Amplifex Diene reagent workflow

Derivatization and sample preparation workflows are quick (30 minute incubations), simple (one-step reactions), reproducible, robust, and high-yielding.



Part Number

Kit Name/ Description


Amplifex Diene Reagent Kit
Contains a protocol, 1 tube of Amplifex Diene reagent, and 1 tube of diluent. Each tube contains enough reagent to complete 50 reactions. This kit replaces 5027600.


Amplifex Diene Sample Prep Starter Kit
Contains hardware components required for Amplifex Diene sample prep. Includes 24-pack C-1000 cartridges, 24-pack S-500 cartridges, 24-pack cartridge adapters, 24 unit rack, and protocol.


Amplifex Diene Cartridge Kit
Contains cartridges for Amplifex Diene sample prep. Includes 24-pack C-1000 cartridges, 24-pack S-500 cartridges, and protocol.


Amplifex Prep Cartridge Adapters, 24-Pack
Replacement cartridge adapters (24-pack) for Amplifex Diene sample prep.


Amplifex Sample Prep 24 Unit Rack
24 unit rack for Amplifex Diene sample prep.