ZenoTOF 7600
mass spectrometer

Be Extraordinary

A powerful leap in proprietary innovation, this high-resolution accurate mass system combines the power of Zeno trap pulsing with EAD fragmentation technology (electron activated dissociation) to uncover structural information, previously inaccessible, and drive the limits of quantification achievable with accurate mass.

ZenoTOF 7600 system

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Welcome to the Zeno revolution.

Driven by the power of the Zeno trap coupled with EAD fragmentation technology, this fragment-centric revolution unlocks sensitivity gains allowing you to uncover new information for certainty in your results to make better-informed decisions, faster.

Detect up to 20x more ions in every experiment and access a spectrum of tuneable fragmentation techniques to unlock new perspectives for every molecule, in every experiment.

Zeno opens the door to a ‘fragment centric revolution’
Maarten Dhaenens Ghent University, Belgium

The Zeno revolution is now...

Zeno trap and EAD. A powerful combination of unparalleled MS/MS sensitivity and a step-change in fragmentation technology. Together they provide the ability to acquire key MS/MS features needed to:

  • Characterize large molecules including post-translational modifications
  • Elucidate positional isomers on small molecules and lipids
  • Identify and quantify proteins and peptides at unparalleled speed
Overcome QTOF MS/MS duty cycle deficiencies

Overcome QTOF MS/MS duty cycle deficiencies

  • >90% ions injected into the TOF
  • Sensitivity gains of up to 5-20X with Zeno trap pulsing
  • Identify and quantify low abundance species
Tunable fragmentation of all molecule types

Tunable fragmentation of all molecule types

Utilize controlled electron activated dissociation (EAD)
MS/MS scan rates of up to 133Hz

MS/MS scan rates of up to 133Hz

Improved DDA and High resolution MRMHR

EAD

Electron activated dissociation (EAD)

EAD allows for a range of free electron based fragmentation mechanisms within one device. The ability to tune electron kinetic energy within an EAD experiment extend the utility of the approach to all molecules type from singly charged small molecules to large multiply charged proteins.

EAD 5ev EAD 7ev EAD 9ev

Biomolecule analysis

Protein based therapeutics come with a rich assortment of varying structures. Those modifications exist in complex heterogeneous mixtures.

CID has been used extensively to determine the structure of biomolecules but full characterization can be difficult without further sample processing e.g. digestions.

Low energy EAD fragmentation enables characterization of intact biomolecules via top down approaches allowing researchers to rapidly identify and confirm key structural attributes.

Technical note: Qualitative flexibility combined with quantitative power Open link

Post-translational modifications

When tuned to higher kinetic energies, EAD has the capability to fragment post-translationally modified peptides such as phosphopeptides and glycopeptides whilst retaining critical MS/MS information for both identification and localisation of PTMs. Unlike other electron-based fragmentation techniques, this can be achieved in conjunction with fast scan speeds and in a reproducible, consistent manner.

The EAD cell brings fresh insight and completes the picture for biomolecule characterization. EAD, fragmentation of large multiply-charged produces those differential C & Z ions to garner those critical insights.

Technical note: A new electron activated dissociation (EAD) approach for comprehensive glycopeptide analysis of therapeutic proteins Open link Technical note: Tuneable electron activated dissociation (EAD) MS/MS to preserve particularly labile post-translational modifications Open link

Small molecules

CID fragmentation of small molecules can produce limited and/ or non-specific MS/MS information, giving rise to challenges in identification or lack of specificity in the development of quantitative assays.

High-energy EAD fragmentation can produce highly specific MS/MS information on small molecules such as endogenous metabolites or the products of drug metabolism. This enables confident, unambiguous identification and highly specific MS/MS-based quantification.

Technical note: Complete structural elucidation of lipids in a single experiment using Electron-Activated Dissociation (EAD) Open link

Zeno trap

The next era of sensitivity for accurate mass

Ions are accumulated in the Zeno trap before being pulsed rapidly into the TOF, meaning we can detect up to 20x more ions.

Consequently, each TOF experiment contains more useful MS/MS information, particularly on lower abundance species that were previously undetectable, introducing our customers to a new sensitivity revolution.

Without Zeno trap pulsing

With Zeno trap pulsing

Use this slider to see the impact of Zeno trap pulsing on this interactive graphic.

Rare quantifiable data becomes your everyday

Zeno DDA EAD/ CID

Zeno DDA EAD/ CID

Fresh perspective and certainty.

SWATH Acquisition

SWATH acquisition

New depth of rare data.

Zeno MRMHR

Zeno MRMHR

High sensitivity and specificity.

A new data landscape enabled by Zeno

Zeno DDA (CID/EAD)

EAD technology allows you to fine tune the electron energy, accessing multiple fragmentation mechanisms that can be applied to large molecules, peptides, lipids and small molecules.

  • 40% more proteins identified using Zeno MS/MS
  • Characterization of lipids in a single experiment
  • Comprehensive coverage and localized glycans in a single run

SWATH acquisition

The eternal dataset. SWATH acquisition delivers comprehensive data-independent acquisition that can be used to quantify every observable species in a sample.

  • SWATH acquisition for higher throughput proteomics

Zeno MRMHR

Zeno trap pulsing on demand gives the ability to detect lower abundance ions with those in abundance at the same time driving the limits of quantification achievable with accurate mass.

  • Zeno MRMHR unlocks new levels of sensitivity

A new data landscape enabled by Zeno

Explore the ZenoTOF 7600

H: 112cm W: 67cm L: 142cm
The system exterior
ION Sources
System - Exterior USP (ION)

Ion Sources

Advanced source designs that limit contamination for maximized uptimes with enhanced usability features from a simplified engineering design built from the legacy of the Turbo V source. Coupled with the calibration delivery system for fast, automated calibrations across the flow rate ranges.

Footprint
System - Exterior USP (Footprint)

Footprint

Reliable and robust accurate mass benchtop MS system. Requiring very little lab space, occupying occupies less lab space than any other HRMS on the market today.

H: 112cm W: 67cm W: 142cm
Software
System - Exterior USP (Software)

Software

Powered by SCIEX OS mass spectrometry software, intuitive algorithms and automation enable you to make informed decisions quickly. Transform your LC-MS/MS workflows with remarkable quantitative usability, efficiency, and integrity that streamlines your entire lab.

ION Path
System - Exterior USP (ION)

Ion Path

Designed to be powerful and versatile delivering enhanced performance for qualitative and quantitative workflows.

Calibration delivery system
System - Exterior USP (ION)

Calibration delivery system

Designed with a new calibration solution that automates mass calibration, and ensures mass accuracy of the system is maintained throughout acquisition.

Order Calibration Standards Order Calibration Standards

Ion Sources

Advanced source designs that limit contamination for maximized uptimes with enhanced usability features from a simplified engineering design built from the legacy of the Turbo V source. Coupled with the calibration delivery system for fast, automated calibrations across the flow rate ranges.

Footprint

Reliable and robust accurate mass benchtop MS system. Requiring very little lab space, occupying occupies less lab space than any other HRMS on the market today.

H: 112cm D: 142cm W: 67cm

Software

Powered by SCIEX OS mass spectrometry software, intuitive algorithms and automation enable you to make informed decisions quickly. Transform your LC-MS/MS workflows with remarkable quantitative usability, efficiency, and integrity that streamlines your entire lab.

Ion Path

Designed to be powerful and versatile delivering enhanced performance for qualitative and quantitative workflows.

Calibration delivery system

Designed with a new calibration solution that automates mass calibration, and ensures mass accuracy of the system is maintained throughout acquisition.

Order Calibration Standards Order Calibration Standards
The System Exterior
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The System interior
EAD cell
System - Interior USP (EAD Cell)

EAD cell

Highly tunable electron activated dissociation (EAD) allows for a range of free electron- based fragmentation mechanisms within one device.

TOF
System - Interior USP (EAD cell)

TOF

N-optic TOF design provides optimal mass accuracy and resolution without compromising sensitivity.

Heated TOF path & drone heaters
Mass Range: 40 to 40 kDa in TOF
Resolution: ≥ 42k at m/z 956
MS/MS Speed: 133Hz
Mass Accuracy: <2 ppm RMS Ext., <1 ppm RMS
Int Positive & Negative LDR:>5 orders Inter-scan LDR

Zeno trap
System - Interior USP (EAD Cell)

Zeno trap

Improved MS/MS duty cycle gain with 5-20X gain in MS/MS sensitivity coupled with either EAD or CID fragmentation

Q0 Design
System - Interior USP (EAD Cell)

Q0 Design

Improved ion optics design for ion capture and transmission, with easy access for maintenance.

LINAC collision cell
System - Interior USP (EAD Cell)

LINAC collision cell

High drive frequency collision cell provides better ion transmission, higher duty cycle, and improved resolution by focusing ions prior to entering TOF.

EAD cell

Highly tunable electron activated dissociation (EAD) allows for a range of free electron- based fragmentation mechanisms within one device.

TOF

N-optic TOF design provides optimal mass accuracy and resolution without compromising sensitivity.

Heated TOF path & drone heaters
Mass Range: 40 to 40 kDa in TOF
Resolution: ≥ 42k at m/z 956
MS/MS Speed: 133Hz
Mass Accuracy: <2 ppm RMS Ext., <1 ppm RMS
Int Positive & Negative LDR:>5 orders Inter-scan LDR

Q0 Design

Improved ion optics design for ion capture and transmission, with easy access for maintenance.

Zeno trap

Improved MS/MS duty cycle gain with 5-20X gain in MS/MS sensitivity coupled with either EAD or CID fragmentation

LINAC collision cell

High drive frequency collision cell provides better ion transmission, higher duty cycle, and improved resolution by focusing ions prior to entering TOF.

The System Interior
Zoom in
Zoom out

Flow rate

Flow rate
Flow rate
Turbo V source

Turbo V source

The SCIEX Turbo V source with a refined source design that limit contamination for maximized uptimes and providing the high- sensitivity across and wide range of flow rates (5 µL/min- 3 mL/min) and compound class coverage with quick interchange of modes between APCI and ESI.

Optiflow source

Optiflow source

The robustness and simplicity you’ve come to expect from traditional analytical flow with Turbo V can now be experienced in your high sensitivity microflow workflows. Intelligent probe and electrode design eliminates manual adjustments and minimizes source optimization for the 1 -200 µL/min workflows.

Optiflow Turbo V source

Optiflow Turbo V source

Uniquely allow the user to switch to nanoflow regimes for the highest sensitivity. Simple, plug-and-play nanoflow capability takes the hassle out of nanospray applications. Integrated column oven, nanoflow probe and electrodes allow easy setup of nanoflow applications with no need for optimization.

Resource Library

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  • Pharma Pharma Pharma
  • life-science lice-science Life Science Research
  • Biopharma Biopharma Biopharma
  • Food Testing Food Testing Food Testing
  • Forensics Forensics Forensics

Technology Overview Qualitative flexibility combined with quantitative power

ZenoTOF 7600 system technical overview, providing an overview of the hardware innovations and some application examples. Highlights include increased sensitivity with Zeno MS/MS, with > 90% duty cycle across the entire MS/MS mass range, and electron activated dissociation (EAD) for tunable free electron-based dissociation for added flexibility during compound identification.

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  • Pharma Pharma Pharma
  • life-science lice-science Life Science Research
  • Biopharma Biopharma Biopharma

Tunable EAD MS/MS to preserve particularly labile PTMs

This technote presents site-localization of malonylated peptides using the SCIEX ZenoTOF 7600 system. Determining the identity and the precise location of a PTM is important to fully characterize function. Electron activated dissociation was investigated for the characterization of labile PTMs, and Zeno trapping for significant sensitivity gains.

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  • Biopharma Biopharma Biopharma

Characterization of Duranavir drug metabolites with EAD fragmentation

The differentiation of drug metabolites is important because each can exhibit different toxicities. Here, we investigate the in vitro metabolism of Duranavir with the ZenoTOF 7600 system, comparing CID and EAD fragmentation techniques. The results show confident assignment and differentiation of O- and N-glucuronide conjugates.

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  • life-science lice-science Life Science Research
  • Pharma Pharma Pharma
  • Biopharma Biopharma Biopharma

Analysis of metabolomics SWATH® Acquisition data using a cloud-based solution

In this technote, the OneOmics suite was used to process a SWATH acquisition data set acquired with the SCIEX ZenoTOF 7600 System, to quantify differences in metabolites found in ZDF and healthy rat urine.

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  • life-science lice-science Life Science Research
  • Pharma Pharma Pharma
  • Biopharma Biopharma Biopharma

Impact of increased MS/MS sensitivity on the untargeted metabolomics workflow

High sensitivity, high resolution, and fast acquisition rates are critical for obtaining very good metabolite identifications in complex matrices. This technote presents how the Zeno MS/MS on the ZenoTOF 7600 system was explored in rat urine. The large increases in MS/MS sensitivity resulted in a 15% improvement in identified metabolites.

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  • Pharma Pharma Pharma
  • life-science lice-science Life Science Research
  • Biopharma Biopharma Biopharma

Targeted metabolomics in rat urine using Zeno MS/MS

In this technote, the targeted MRMHR assay has been developed for a small panel of metabolites in urine. The Zeno MS/MS provided a 13-fold average increase in MS/MS sensitivity, providing high-quality, full-scan MS/MS data for confident metabolite identification, higher sensitivity quantification, and the use of smaller sample volumes.

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  • Food Testing Food Testing Food Testing

Pesticides in food with Zeno MRMHR

Highly sensitive pesticide detection with a high degree of selectivity and identification confidence using Zeno MRMHR to quantify low levels of pesticides in olive oil and various fruits and vegetables to meet global regulations.

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  • Pharma Pharma Pharma
  • life-science lice-science Life Science Research
  • Biopharma Biopharma Biopharma

Complete structural elucidation of lipids in a single experiment using EAD

In this technote, electron-activated dissociation (EAD) on the ZenoTOF 7600 System is used for the complete structural elucidation of glycerophospholipids, sphingolipids, and acylglycerols in a single experiment. In contrast to the collision induced dissociation CID, EAD provides an abundance of unique fragment ions critical for complete lipid characterization.

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  • life-science lice-science Life Science Research
  • Pharma Pharma Pharma
  • Biopharma Biopharma Biopharma

Over 40% more proteins identified using Zeno MS/MS

Collect high-quality MS/MS at very high acquisition rates is key for high numbers of peptide and protein identifications. This technote presents how the ZenoTOF 7600 system delivers a ~5-fold gain in MS/MS sensitivity. Over 40% improvements in protein IDs were observed relative to previous technology for identical sample loadings.

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  • Pharma Pharma Pharma
  • life-science lice-science Life Science Research
  • Biopharma Biopharma Biopharma

Performing large scale targeted peptide quantification using Zeno MS/MS

In this technote, a highly multiplex targeted peptide quantification assay has been developed to explore the quantitative capability of Zeno MS/MS on the ZenoTOF 7600 system. 804 peptides were analyzed in a single time-scheduled MRMHR acquisition using a 20 min gradient, with Zeno MS/MS accumulation times down to 10 msec.

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  • Pharma Pharma Pharma
  • life-science lice-science Life Science Research
  • Biopharma Biopharma Biopharma

Differentiation of leucine and isoleucine using Electron Activation Dissociation (EAD)

An unambiguous differentiation of leucine (Leu) and isoleucine (Ile) in peptides derived from a monoclonal antibody (mAb) therapeutic is achieved utilizing a new fragmentation type based on EAD and featuring the SCIEX ZenoTOF 7600 system and Protein Metrics Inc. software.

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  • Biopharma Biopharma Biopharma

Peptide mapping of biopharmaceuticals utilizing EAD

Automated characterization of challenging post-translational modifications, the confirmation of amino acid isomers and a streamlined disulfide mapping approach is routinely achieved using EAD as part of a fully automated DDA workflow with the SCIEX ZenoTOF 7600 system and Protein Metrics software. .

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  • Biopharma Biopharma Biopharma

Confirmation of disulfide linkages in adalimumab using EAD

Demonstrates a new fragmentation type based on EAD for regular and advanced confirmation of disulfide-linked peptides from a commercial biotherapeutic mAb in one injection. Leveraging the SCIEX ZenoTOF 7600 system using an untargeted 10 Hz DDA method and interpreted with Protein Metrics Inc. software.

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  • Biopharma Biopharma Biopharma

Workflow of intact and subunit mass analysis for monoclonal antibodies (mAb)

Comprehensive, yet straightforward, workflow of intact and subunit analysis for NISTmAb is reported using the SCIEX ZenoTOF 7600 system combined with BPV Flex software 2.1. Alongside mass confirmation, different post-translational modifications (PTMs) such as glycosylation and terminal modification are characterized.

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  • Biopharma Biopharma Biopharma

Evaluation of biotherapeutic sequence coverage using EAD

Demonstrates that novel EAD is highly comparable to traditionally used collision induced dissociation (CID). Featuring the SCIEX ZenoTOF 7600 system using EAD and Protein Metrics Inc. software. This solution takes peptide mapping workflows to a new level, one previously thought unachievable.

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  • Biopharma Biopharma Biopharma

Characterization of an antibody-drug-conjugate (ADC) using EAD

Characterization of lysine-linked drugs in an ADC sample with the SCIEX ZenoTOF 7600 system and Protein Metrics Inc software. Alternative fragmentation using EAD was leveraged for detailed structural information on the drug and linker, along with obtaining peptide backbone information.

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  • life-science lice-science Life Science Research
  • Biopharma Biopharma Biopharma

A EAD approach for comprehensive glycopeptide analysis of therapeutic proteins

Demonstrates the advantage of EAD, unique to the SCIEX ZenoTOF 7600 system, over traditionally used collision induced dissociation (CID). Streamlined, advanced glycopeptide characterization and localization in peptide mapping characterization is achieved in one injection, with Protein Metrics Inc. processing software.

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  • Biopharma Biopharma Biopharma

Peptide level characterization of a multispecific monoclonal antibody (mAb)

Identification and localization for a variety of PTMs with the SCIEX ZenoTOF 7600 system using the Zeno trap with electron activated dissociation (Zeno EAD) as part of a fully automated data-dependent acquisition (DDA) workflow, with automated data analysis using Protein Metrics Inc. software..

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  • Biopharma Biopharma Biopharma

Characterization of oligonucleotides and related impurities

This technical note describes fast and accurate characterization of oligonucleotides and their impurities using the SCIEX ZenoTOF 7600 system and Molecule Profiler software. It demonstrates confident identification, relative quantification and full sequence coverage at levels as low as 0.3% (w/w).

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  • Biopharma Biopharma Biopharma

Metabolite ID and relative quantification of oligonucleotides in plasma

This technical note describes the identification, relative quantification at levels as low as 0.1% (w/w) and structural confirmation at levels down to 1% of the chain-shortened metabolites of a phosphorothioated oligonucleotide using the SCIEX ZenoTOF 7600 system and Molecule Profiler software.

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  • Pharma Pharma Pharma
  • Biopharma Biopharma Biopharma

Enhanced sensitivity for improved HRMS peptide quantification

The ZenoTOF 7600 system improves MS/MS sensitivity by enhancing the duty cycle with Zeno trap pulsing. Here, we combine the use of Zeno trap with the summation of abundant fragment ions for peptide quantification. The results show a significant average of 5-fold improvement in LLOQ.

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  • Pharma Pharma Pharma
  • Biopharma Biopharma Biopharma

Enhanced HRMS performance for improved peptide quantification

Here, improved selectivity and greater sensitivity were achieved for peptide quantification in a complex matrix using the ZenoTOF 7600 system featuring the Zeno trap.

image description
  • Pharma Pharma Pharma
  • life-science lice-science Life Science Research
  • Biopharma Biopharma Biopharma

PTM site localization and isomer differentiation of phosphorylated peptides

In this technote, the tunable electron activated dissociation (EAD) for phosphopeptide analysis and confident site-localization was evaluated on the ZenoTOF 7600 system. The combination of Zeno MS/MS with EAD provided high-quality MS/MS for full characterization of the peptide including clear differentiation of the two phosphopeptides isomers studied.

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  • Forensics Forensics Forensics

NSO and metabolites detection with the 7600

Low level identificaion of NSOs on the 7600 with spectral library matching. Monitor low levels of potent NSO and their metabolites in poly drug intake scenarios.

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