Choosing the Peak Selection Criteria when Using SCIEX OS AutoPeak Integration Algorithm

For research use only. Not for use in diagnostic procedures.

Answer

There are four types of peak selection criteria that can be applied to a SCIEX OS quantitation method: Expected RT, IS, Largest, and Group. These can be selected when working with the AutoPeak integration algorithm under Project Default Settings under the Projects menu in the Analytics mode.

• Use Expected RT when the retention time (RT) is known and you are not using stable-labeled isotopes for your internal standard (IS). Only one fragment per analyte is allowed.
• Use Largest when the RT is not known and you are not using stable-labeled isotopes for the IS. Only one fragment per analyte is allowed.
• Use IS when using a stable-labeled IS for each analyte.
• Use Group to monitor two or more fragment ions from the same precursor ion.