confidence in your proteomics research results, without compromise
Proteomics research
Immerse yourself into the depths of the proteome
Unlock new, precise perspectives on the proteome
New technologies enable more research applications using proteomics by maximising the depth of data. MS-based proteomics offers the unique advantage to comprehensively analyze proteins and their changes, including post-translational modifications (PTMs), to give a global interpretation of biological systems.
This analytical capability has pushed the scientific community to develop new instrument features and novel workflows for increased versatility to enable applications that were previously sought impossible.
POWER UP YOUR LABORATORY
The Power of Precision.
Proteomics research
Confidence
from discovery to translational research
and beyond
Maximize the depth of your data...
Unlock new, precise perspectives on the proteome
New technologies enable more research applications using proteomics by maximising the depth of data. MS-based proteomics offers the unique advantage to comprehensively analyze proteins and their changes, including post-translational modifications (PTMs), to give a global interpretation of biological systems.
This analytical capability has pushed the scientific community to develop new instrument features and novel workflows for increased versatility to enable applications that were previously sought impossible.
Overview
In pursuit of nanoscopic discoveries for big world impact
The inherent complexity of proteomes, with their variability and dynamic range, have dictated the need to maximize discovery and quantification capabilities without compromising one over the other.
In life science research, “shotgun” or bottom-up proteomics (tandem LC-MS/MS) is a key method used to comprehensively analyze proteins and their changes - including post-translational modifications (PTMs) - to give a global interpretation of biological systems. In general, there are two main approaches to generate tandem mass spectra, both which are supported by SCIEX solutions including: data dependent acquisition (DDA), also known as information dependent acquisition (IDA), and SWATH data independent acquisition (DIA).
Data/information dependent acquisition (DDA/IDA)
The speed and sensitivity of the ZenoTOF 7600 system, which uses using the Zeno trap at scan speeds in excess of >133 Hz, delivers the highest-quality MS/MS data during data-dependent acquisition (DDA), even on low-abundance proteins.
SWATH DIA delivers precise, extensive, label-free quantification that drives biomarker discovery and identification of differentially expressed proteins. The ability to generate MS/MS data on all observable species at speed enables this approach to simultaneously identify and quantify thousands of proteins, even during short run times and with small sample loads.
discovery proteomics
Confident identification and quantification
Move research from discovery to translational, and beyond, with more confident and significant biomarker signatures to uncover insights in their biology and mechanism of action. Using the latest technologies from SCIEX, a whole new level of proteomic depth can be discovered by uncovering unique fragmentation patterns and low abundant species.
Next-generation proteomics
Protein profiling and characterization
From post-translational modifications (PTMs) to full proteome profiling and characterization, the dynamic nature of proteins make them very challenging to analyze. Researchers require advanced technology to study protein dynamics reliably and reproducibly to determine profile protein structure changes, including PTMs profiling. SCIEX solutions allow researchers to reliably and reproducibly profile protein structure changes using the latest innovations in fragmentation technology to yield rich and informative MS/MS. Benefit from 100% sequence coverage in a single injection with and identify low-level PTMs faster than ever before.
targeted proteomics
Monitor and quantify proteins of interest with absolute precision
Verification and validation of protein biomarkers require precise, targeted quantification across large numbers of samples to enable translation into clinical research. SCIEX solutions for targeted proteomics are driven by renown MS/MS sensitivity and precision further enhanced by Zeno trap and Zeno SWATH DIA technology.
Going library-free for protein identification using Zeno SWATH DIA and in silico-generated spectral libraries
This Zeno SWATH DIA workflow, using an in silico-generated library processing with DIA-NN software, was superior to the traditional shotgun proteomics approach using Zeno DDA for the identification of large numbers of proteins from complex samples, and agreed with two commonly used experimentally generated libraries.
Pharma
Biopharma
Life Science
New
Zeno MS/MS with microflow chromatography powers the Zeno SWATH DIA workflow to quantify more proteins
As quantitative proteomics evolves, larger biological cohorts are being studied, often with precious samples. This creates the need to acquire data faster and with smaller amounts. Zeno SWATH DIA, with Zeno MS/MS and microflow LC, has the potential to significantly enhance core quantitative proteomics workflows.
Pharma
Biopharma
Life Science
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High-throughput quantitative proteomics using Zeno SWATH DIA and the Evosep One system
Proteins and peptides are important in translational research to understand biological function. This method achieves 5-6x more MS/MS sensitivity, resulting in this DIA approach surpassing DDA for protein identifications and quantification in complex matrices.
Pharma
Biopharma
Life Science
New
Nanoflow Zeno SWATH DIA for high-sensitivity protein identification and quantification
Nanoflow chromatography is used in workflows to obtain the highest sensitivity. High-quality chromatographic separations are important, as good peak shape and peak resolution can reduce ion suppression and allow the MS system to sample as many unique peptides as possible.
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Biopharma
Life Science
New
Protein quantification at subnanogram loads using Zeno SWATH DIA and nanoflow chromatography
DIA has emerged as a comprehensive workflow for label-free quantitative proteomics, allowing for the acquisition of MS/MS spectra on all detectable peptides. Zeno SWATH DIA in the ZenoTOF 7600 system leverages Zeno trap activation for 5-6x increases in peptide MS/MS sensitivity in SWATH DIA workflows.
Pharma
Biopharma
Life Science
New
Large scale protein identification using microflow chromatography on the ZenoTOF 7600 system
Generation of spectral libraries is often thought of as a time consuming procedure, but in reality, they can be generated quickly. Using microflow chromatography with fast MS/MS acquisition on a QTOF, then searching the data in the cloud can enable library generation in <48 hrs.
Differential protein expression analysis using isobaric labelling reagents and mass spectrometry requires instrumentation that can acquire high resolution MS/MS at high acquisition rates with excellent sensitivity for good quantification. The ZenoTOF 7600 system provides large improvements in MS/MS sensitivity.
Pharma
Biopharma
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Over 40% more proteins identified using Zeno MS/MS
Proteomics samples are some of the most complex samples analyzed by mass spectrometry. The ZenoTOF 7600 system delivers a ~5-fold gain in MS/MS sensitivity when analyzing peptides, so this study investigates the impact of this large sensitivity gain on identification rates.
Pharma
Biopharma
Life Science
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Large-scale, targeted, peptide quantification of 804 peptides with high reproducibility, using Zeno MS/MS
A highly multiplex targeted peptide quantification assay has been developed to explore the quantitative capability of Zeno MS/MS. Using a mixture of 804 heavy labeled synthetic peptides dosed into plasma, the increase in peptide sensitivity due to Zeno MS/MS was found to be 5.6 fold.
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New
PTM site localization and isomer differentiation of phosphorylated peptides
Specific location of phosphorylation sites on proteins is important in fully understanding their role in cellular processes. Here, the use and benefits of tunable electron activated dissociation (EAD) for phosphopeptide analysis and confident site-localization was evaluated on the ZenoTOF 7600 system.
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Biopharma
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Tunable electron activated dissociation (EAD) MS/MS to preserve particularly labile PTMs
Determining the identity and the precise location of a post-translational modification (PTM) on a protein is important to fully characterize function. Here, the utility of electron activated dissociation on the SCIEX ZenoTOF 7600 system was investigated for the characterization of labile PTMs.
Pharma
Biopharma
Life Science
New
Highly sensitive quantification of proteins from the SARS-CoV-2 antigen in nasopharyngeal swab samples
Investigation of the nasopharyngeal swabs use in a saline matrix for the quantification of SARS-CoV-2 proteins using LC-MS/MS. The gains in sensitivity attained by moving the assay onto the 7500 system and reducing matrix effects by changing swab storage medium were characterized.
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Biopharma
Life Science
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Reproducible targeted peptide profiling using highly multiplexed MRM assays
Using the scheduled MRM algorithm to build highly multiplexed peptide MRM methods enables a higher number of MRMs to be monitored concurrently while maintaining optimal dwell and cycle times. The 7500 system for large scale targeted peptide quantification was evaluated using microflow chromatography.
Pharma
Biopharma
Life Science
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Improved LC-MRM quantification sensitivity for cyclic peptides from the natriuretic peptide family
Cyclic peptides have been identified as important therapeutic modalities. This is related to their stability in blood and their potential for oral dosing. LC-MS method development to quantify trace levels of cyclic peptides in biological matrices has remained challenging.
Pharma
Biopharma
Life Science
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Significant gains in quantitative sensitivity using microflow chromatography
Increasingly, microflow chromatography is being adopted in applications because of the “sweet spot” it occupies between increased sensitivity and solid robustness.
Pharma
Biopharma
Life Science
New
Enhancing the sensitivity of peptide quantification for the targeted HCPs analysis
Host cell proteins (HCPs) are a major class of process-related impurities that accompany a recombinant biotherapeutic product during production. As their levels impact the potential toxicity and efficiency of the therapeutics, there are significant requirements for the quantitative measurement of HCPs across the entire development paradigm.