How To Ramp Parameters in SCIEX OS Software during Compound Optimization

For research use only. Not for use in diagnostic procedures.

Answer

When using SCIEX OS software to optimize compounds that are being infused using a syringe pump, it is possible to ramp individual parameters such as collision energy (CE), entrance potential (EP), and collision cell exit potential (CXP), and Q0 dissociation (Q0D). This process may be preferred to guided MRM optimization in the event that a more manual and controlled optimization is desired.

To optimize compounds, select the MS Method pane in SCIEX OS and open a new MRM method. In the top right corner, select the dropdown options menu and then select Ramp (see image below).


The Ramp Compound Parameters window will open; select the parameter to be optimized along with the range and step size. The acceptable ranges for compound parameters are as follows:

• Collision energy (CE): 5 to 180 V
• Entrance potential (EP): 2 to 15 V
• Collision cell exit potential (CXP): 0 to 55 V
• Q0 dissociation (Q0D): -10 to 200 V

In order for Q0 Dissociation to be an option in the drop down it has to be checked in the MRM method.


Finally, check the box to apply the ramping to the MRM method and then click OK as shown below.


While the method is running, a message will be displayed, "Ramping:ON" at the bottom right of the method, indicating that the ramping of the selected compound parameter is underway. If ramping is ON, the method duration and the compound parameter value in the table of the method itself will be superseded by the duration and value of the ramping.