Why SCIEX OS does not create a .wiff file with certain configuration of acquisition mode ?


Date: 04/10/2024
Categories: SCIEX OS

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For research use only. Not for use in diagnostic procedures.


Answer

Here is the list of conditions for which there is no creation of a wiff file, with only a wiff 2 for SCIEX OS.

The following exceptions apply:

Nominal Mass

  • Overlapping scheduled experiment (e.g. experiment 1 = 0 to 2 min, experiment 2 = 1 to 2 min, where the two experiments are overlapping from 1 to 2 min)
  • Looped IDA (e.g. MRM+ IDA, IDA + IDA) 
  • 3 or more MRM experiments (e.g. MRM + MRM + MRM) 
Accurate Mass:
  • Scheduled experiment (both overlapping and/or non-overlapping)
  • Q1 
  • Combinations of IDA + (s)MRMHR
  • Combinations of (s)MRMHR + IDA
  • Combinations of IDA + IDA
  • Combinations of IDA + SWATH
  • Combinations of SWATH +IDA
  • Combinations of SWATH +(s)MRMHR
  • Combinations of (s)MRMHR +SWATH