In untargeted metabolomics, the quest for discovery, the quest to find something that has not been found before is a true struggle of desire and effort versus reward. Potentially the most challenging approach to metabolomics and biomarker discovery lies in the complex interpretation of “differential features” and how to convert lists of such features into metabolite identifications and contextualize them in biology.
Approaches to untargeted metabolite profiling generally involve statistical treatment of LC-MS data to generate a list of features that can be potentially identified. Often this can involve multiple injections of a sample to ensure information can be collected on features of interest. Ultimately the nature of a metabolomics dataset is such that often no identity can be assigned to a given feature due to it being an artifact or simply that its structure cannot be inferred from the data.
SCIEX solutions for untargeted and discovery metabolomics focus on generating comprehensive datasets containing large amounts of information through unique, modern approaches like SWATH acquisition, that can be curated repeatedly using comprehensive statistics, along with tools developed by our collaborators aimed at identifying metabolites of interest through simplification and deconvolution of the data.
Give yourself and your samples the best chance, without compromising on depth of coverage. Combine comprehensive data acquisition strategies with advanced data processing pipelines to deconvolute and focus the data generated to increase your chances of finding novel biologically-relevant differential metabolites.
Advanced data processing enabled by XCMS Plus software. A powerful in-house solution to unknown metabolite discovery using the most cited metabolomics software.
SWATH acquisition captures MSMS data for every observable species. See how MS DIAL software allows comprehensive data extraction from this novel approach.
Over 75% of your feature data can be artifacts. Concentrate on the peaks that matter with SWATH acquisition and isotope ratio outlier analysis (IROA).
Making breakthrough discoveries requires exceptional technological innovations. Combining the speed, sensitivity, and dynamic range of the new ZenoTOF 7600 system and the comprehensive quantitative MS/MS capabilities of SWATH data independent acquisition with novel data processing gives you the tools needed to drive faster discovery of new disease markers and therapies.
In an application where data quality, completeness and interpretation are paramount, discover the most modern approaches to turning differential feature lists into real, tangible metabolite discoveries.
The M5 MicroLC system is the perfect balance of sensitivity and robustness, giving you the potential to optimize your crucial workflows, today and into the future.
The most comprehensive approach to metabolite profiling. Data-independent MS/MS on everything with no data gaps.
Qualitative flexibility combined with quantitative power: the ZenoTOF 7600 system unlocks new, precise perspectives on the metabolome.
Create, manage and curate MS/MS libraries. SCIEX Accurate Mass Metabolite Library provides an advanced starting point.