Sensitivity: more proteins identified, more proteins quantified

Experience the 5–6x increase in peptide MS/MS sensitivity that the Zeno trap provides, all while maintaining the same critical performance specifications you have come to rely on.

Reproducibility: achieve excellent inter-day precision of quantification

As sample loading decreases, run-to-run variability in protein identification and quantification can become more extreme. Because of the data-independent nature of Zeno SWATH DIA, you can stop worrying about poor reproducibility when using low sample loadings.

Higher productivity: proteome coverage and quantitative reproducibility in a single run

Fully leverage the sensitivity gains provided by the Zeno trap to get even more proteins quantified from your nanoflow chromatography on low amounts of proteomic sample. With Zeno SWATH DIA, you get 4,000–6,000 quantified proteins in a single 1-hour run.

Go library-free: eliminate the need to build spectral libraries

By using an in silico-generated library, you can run faster with a more streamlined workflow for protein identification and quantification while retaining a depth of coverage that is similar to experimental libraries.

Going library-free for protein identification using Zeno SWATH DIA and in silico-generated spectral libraries

This Zeno SWATH DIA workflow, which uses DIA-NN software and an in silico-generated library for processing data, outperformed the traditional shotgun proteomics approach that uses Zeno DDA for the identification of large numbers of proteins from complex samples. The Zeno SWATH DIA workflow also demonstrated high agreement with two commonly used experimentally generated libraries.

Zeno MS/MS with microflow chromatography powers the Zeno SWATH DIA workflow to quantify more proteins

As quantitative proteomics evolves, larger biological cohorts are being studied, often using precious samples. This creates the need to acquire data faster and using smaller amounts. Using Zeno SWATH DIA with Zeno MS/MS and microflow LC has the potential to enhance core quantitative proteomics workflows significantly.

High-throughput quantitative proteomics using Zeno SWATH DIA and the Evosep One system

In translational research, proteins and peptides are important for understanding biological function. The Zeno trap provides a 5–6x more peptide MS/MS sensitivity, resulting in a powerful DIA workflow that is compatible with fast quantitative proteomics.

Protein quantification at subnanogram loads using Zeno SWATH DIA and nanoflow chromatography

Zeno SWATH DIA in the ZenoTOF 7600 system leverages Zeno trap activation for a 5–6x increase in peptide MS/MS sensitivity. This high sensitivity opens the door to performing label-free proteomics on very low amounts of proteomic samples.

Nanoflow Zeno SWATH DIA for high-sensitivity protein identification and quantification

Nanoflow chromatography is used in workflows to obtain the highest sensitivity. High-quality chromatographic separations are important, as good peak shape and peak resolution can reduce ion suppression and allow the MS system to sample as many unique peptides as possible.

Large-scale protein identification using microflow chromatography

Using microflow chromatography with fast MS/MS acquisition and then searching the data in the cloud can enable large-scale protein identification in a short amount of time. Here, using the ZenoTOF 7600 system and two fractionated human cell lines, a very deep DIA library was generated in <48 hours.

Integrating DIA-NN software analysis of SWATH DIA into a cloud processing pipeline

The OneOmics suite is a unified platform that provides researchers with tools for multi-omics data management, compound identification and quantification, statistical analysis and pathway analysis to streamline biomarker discovery studies. This cloud-powered solution also enables rapid and secure sharing of results with collaborators. Here, a complete processing workflow for proteomics analysis is presented using Zeno DDA and Zeno SWATH DIA data acquired on the ZenoTOF 7600 system.

Flexibility, speed, and throughput for high proteome coverage

This work demonstrates the use of Zeno SWATH DIA on the ZenoTOF 7600 system coupled with the Evosep One system to achieve quantitative proteomics on both HeLa cell and nondepleted human plasma digests. The sensitivity of the ZenoTOF 7600 system is demonstrated at different throughput levels. At 30 samples-per-day (SPD), >8,000 protein groups were identified from 200 ng of HeLa digest, while at 500 SPD, >270 protein groups could be identified from 50 ng of non-depleted human plasma.

ZenoTOF 7600 system

The ZenoTOF 7600 system is a novel accurate mass spectrometry solution that combines rich MS/MS data with impressive sensitivity, powerful new fragmentation technology and a step-change in data independent acquisition for characterization and quantification.

X500R QTOF system

The X500R QTOF system reduces the complexity of accurate mass spectrometry while maintaining the quantitative capabilities you need for various targeted and untargeted analyses.

OneOmics suite

Use the power and scalability of cloud-based software to extract and visualize mass spectrometry data from multi-omics projects, and share those biological insights widely and easily with collaborators.

Mycotoxins

Analysis of designer forensic drugs

Untargeted metabolomics

Biologic peptide mapping analysis