iMethod™ Application for Azo and Sudan Dyes Version 1.1 for Cliquid® Software

For research use only. Not for use in diagnostic procedures.

Quantitate and confirm the presence of Sudan and azo dyes in foods

This SCIEX iMethod™ Application allows you to analyze for 13 azo dyes, 6 Sudan dyes, Dimethyl Yellow and Para Red in spices and sauces accurately in a single analysis.   Included with this method is a Phenomenex LUNA 5 µm C8 column (150 x 2 mm).

To learn more, please view this flyer: A Rapid iMethod™ Application for the Quantification of Azo Dyes in Food

Status: Available Item status information

iMethod Application for Azo and Sudan Dyes Version 1.1  for Cliquid® Software


Just download a method, add your sample, and you're ready to go

iMethod™ Applications for food testing provide instant LC-MS/MS methods, enabling you to generate accurate and precise results faster. Simply download a lab-proven method from the web or a CD into Cliquid® Software and run your sample. All the instrument parameters are downloaded upon installation. Everything you need to start running a test immediately is included: sample preparation protocols, optimized MS and LC parameters, integration parameters, reporting templates, and documentation.

Quantitate and confirm 13 azo dyes, 6 Sudan dyes, dimethyl yellow and Para Red in food

The iMethod™ Application provides a method for the analysis of azo dyes in food including the Sudan dyes (Sudan I, Sudan II, Sudan III and Sudan IV) as well as Para Red, Fast Garnet GBC, Orange II, Rhodamine B and Dimethyl Yellow. According to International Agency for Research on Cancer, azo dyes have been classified as potential carcinogenic substances. As a result, azo dyes are banned as food additives in the European Union.

Samples are prepared by homogenization, centrifugation, extraction, dilution, and examples of procedures are provided with the method. Deuterated internal standards of Sudan I and Sudan IV are added during sample preparation to monitor sample recovery. More in-depth sample preparation, instrument configuration, and setting information is included with this iMethod™ Application, upon purchase.

The SCIEX iMethod™ Application for the analysis of azo dyes is configured for use on the SCIEX 3200 and 4000 Series instrumentation.

The list of available iMethod™ Applications located in the iMethod™ Store is continually updated with the newest user-contributed and  SCIEX lab-proven methods, so you can take immediate advantage of the latest advances. All iMethod™ Applications for food analysis are available for individual purchase or as part of one of our starter packs for food safety testing.

Reduce development time

ActivityUsing an iMethod™ ApplicationTraditional Method Development
Complete Test Protocol DocumentationIncludedWeeks–Months
Sample Prep TechniquesIncluded, with examples and referencesWeeks–Months
Optimized MS/MS Acquisition ParametersIncludedWeeks–Months
LC Instrument Parameters with Retention TimesIncludedDays–Weeks
Integration (Quantitation) ParametersIncludedDays
Reporting TemplatesIncludedDays
LC/MS/MS Library SpectraMay be included. Stand-alone libraries are also available.Weeks–Months
Staff Training – SoftwareSimple Cliquid® Software interface, no additional trainingTraditional MS Software and review procedures – Days
Method ValidationWeeks. Validation templates and support availableWeeks–Months
Complete Test Development and DeploymentWeeks6–12 months